Journal: Cytokine

Article Title: Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34

doi: 10.1016/j.cyto.2012.08.008

Figure Lengend Snippet: Activity of porcine and human recombinant CSF-1 on canine and feline bone marrow progenitor cells. Canine and feline bone marrow cells were cultured with either 10 4 U/ml rh-CSF-1, 300ng/ml porcine E.Coli expressed CSF-1 or no growth factors. By day 5 of differentiation, canine and feline BMDMs with no growth factors were dead (canine A & feline D). By day 5 of differentiation for canine and day 12 for feline, BMDMS were attaching to the culture dish when cultured with either rh-CSF-1 (canine B & feline E) or porcine CSF-1 (canine C & feline F).

Article Snippet: Recombinant mouse and human IL-34 are both commercially available (R&D Systems).

Techniques: Activity Assay, Recombinant, Cell Culture

Journal: Cytokine

Article Title: Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34

doi: 10.1016/j.cyto.2012.08.008

Figure Lengend Snippet: Activity of recombinant human and mouse IL-34 on porcine CSF-1R expressed in Ba/F3 cells. An MTT cell viability was used to assess the biological activity of human and mouse IL-34 on expressed porcine CSF-1R. (A) Both human and mouse IL-34 are biologically active on the porcine receptor. (B) Human IL-34 has demonstrates similar activity to rh-CSF-1 on the porcine CSF-1R in an MTT assay.

Article Snippet: Recombinant mouse and human IL-34 are both commercially available (R&D Systems).

Techniques: Activity Assay, Recombinant, MTT Assay